Analysis of Rabbit Myxomatosis Virus (RMV) by Real-Time PCR Method (CAT#: STEM-MB-3443-LGZ)

Introduction

Myxomatosis is a fatal disease of all breeds of domesticated rabbits caused by myxoma virus, a member of the poxvirus group. Myxomatosis is called “big head” and is characterized by mucinous skin lesions or myxedema of the head. Wild rabbits such as the cottontail (Sylvilagus) and jackrabbits (Lepus) are quite resistant.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.