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Analysis of PAF acetyl-hydrolase (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0478-LGZ)

Introduction

Platelet activating factor (PAF) is a biologically active phospholipid synthesized by stimulation of various cells. PAF is converted to the biologically inactive lyso-PAF by PAF acetylhydrolase (PAF-ah). PAF-AHs are located intracellularly and extracellularly (eg, in the cytoplasm and plasma). Plasma PAF-AH is highly selective for phospholipids with a very short acyl group at the sn-2 position and is associated with lipoproteins.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cardiac Biomarkers, Cardiovascular

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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