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Analysis of FGF-7 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0178-LGZ)

Introduction

Fibroblast growth factor 7 (FGF7) is a secreted protein found mainly in epithelial cells and belongs to the heparin-binding growth factor family. Members of the FGF family have broad mitotic and cell survival activities and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF7 is a highly efficient epithelial cell-specific growth factor, and its mitotic activity is mainly expressed in keratinocytes, but not in fibroblasts and endothelial cells. It may be a major paracrine effect of normal epithelial cell proliferation.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Oncology & Cancer

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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