Analysis of PDE4DIP Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0557-LGZ)

Introduction

Official Full Name: phosphodiesterase 4D interacting protein
Also known as: MMGL; CMYA2
The protein encoded by this gene serves to anchor phosphodiesterase 4D to the Golgi/centrosome region of the cell. Defects in this gene may be a cause of myeloproliferative disorder (MBD) associated with eosinophilia. Several transcript variants encoding different isoforms have been found for this gene.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of CNGA4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2229-LGZ)
Analysis of Jamestown Canyon Virus (JCV) by Real-Time PCR Method (CAT#: STEM-MB-3519-LGZ)
Analysis of Cooperia Spp. by Real-Time PCR Method (CAT#: STEM-MB-2623-LGZ)
Analysis of CSRP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0904-LGZ)
Analysis of SNRPE Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0674-LGZ)
Analysis of Francisella Novicida by Real-Time PCR Method (CAT#: STEM-MB-3585-LGZ)
Analysis of C1orf54 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0263-LGZ)
Analysis of Flos Carthami by Real-Time PCR Method (CAT#: STEM-MB-3960-LGZ)