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Analysis of PDGFR-β (total) (Human) by ELISA (CAT#: STEM-MB-1122-LGZ)

Introduction

Platelet derived growth factor (PDGF) is an alkaline protein stored in platelet α particles. It is a low molecular weight cytokinin. It can stimulate fibroblasts, glial cells, smooth muscle cells and other cells stuck in G0/G1 phase to enter the cycle of division and proliferation. Platelet-derived growth factor PDGF is a peptide regulatory factor that stimulates the growth of connective tissue and other tissue cells discovered in 1974. It is named for its origin in platelets. It exists in the α granules of platelets under normal physiological conditions and is released and activated by disintegrating platelets when blood coagulates. PDGF has the biological activity of stimulating specific cell chemotaxis and promoting specific cell growth. In addition, macrophages, vascular smooth muscle cells, fibroblasts, endothelial cells and embryonic stem cells can also synthesize and release PDGF when tissues are damaged. PDGF can be secreted by macrophages, platelets, infiltrating inflammatory cells, damaged endothelial cells and activated hepatic astrocytes when liver is damaged. It acts in an autocrine or paracrine manner. The binding PDGF is A thermostable glycoprotein with molecular weight of 30KD, which is a dimer formed by the combination of two polypeptide chains A and B connected by disulfide bond.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Neurobiology/Neurodegeneration, Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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