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Analysis of Phenylethanolamine A by ELISA (CAT#: STEM-MB-2247-LGZ)

Introduction

Phenylethanolamine A is a new generation of clenbuterol which is an adrenal receptor agonist known as a neurostimulant. In the late 1980s, it was found to promote the growth of livestock, reduce fat, improve the efficiency of lean meat rate, and was used as a feed additive. However, phenylethanolamine A has been banned since the early 1990s due to a number of poisoning cases and deaths. Phenylethanolamine A rapid detection kit adopts direct competitive ELISA one-step method to detect phenylethanolamine A in urine and meat tissue.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

For food safety.

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Muscle and liver tissue, urine sample, serum, feed
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