Analysis of PPP2R5A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0593-LGZ)

Introduction

Official Full Name: protein phosphatase 2 regulatory subunit B'alpha
Also known as: B56A; PR61A; B56alpha
The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of four major serine/threonine phosphatases involved in the negative control of cell growth and division. It consists of a common heteromeric core enzyme consisting of a catalytic subunit and a constant regulatory subunit in association with multiple regulatory subunits. The B regulatory subunit likely modulates substrate selectivity and catalytic activity. This gene encodes the alpha isoform of the B56 subfamily of regulatory subunits. Alternative transcript variants encoding different isoforms of this gene have been found.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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