Analysis of Presenilin-1 NTF (Human) by ELISA (CAT#: STEM-MB-1137-LGZ)

Introduction

The enzyme gamma-secretase catalyzes the proteolytic cleavage of the membrane to generate amyloid β-peptide from β-amyloid precursor protein. The presenilin (PS) protein is one of four integral membrane protein components of the mature γ-secretase complex. The PS protein itself undergoes endoproteolytic processing to generate stable heterodimers of N-terminal and C-terminal fragments (NTF and CTF, respectively). Here we demonstrate that coexpression of PS1 NTFs and CTFs functionally mimics the expression of full-length PS1 protein and restores γ-secretase activity in PS-deficient mammalian cells. The coexpressed fragments reassociate with each other inside the cell, where they also interact with nicastrin, another γ-secretase complex component.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Neurobiology/Neurodegeneration, Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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