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Analysis of PRKAB2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0588-LGZ)

Introduction

Official Full Name: protein kinase AMP-activated non-catalytic subunit beta 2<br />The protein encoded by this gene is the regulatory subunit of AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of a catalytic alpha subunit and noncatalytic beta and gamma subunits. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthes is fatty acid and cholesterol . This subunit may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific effects. Multiple alternatively spliced transcript variants have been found for this gene.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements