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Analysis of PSD-95 (Human, Mouse, Rat) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0507-LGZ)

Introduction

Postsynaptic density (PSD) is a special structure located in the postsynaptic membrane of the central nervous system, which is a key substance in postsynaptic signal transduction and integration. PSD-95 has no protease activity. It mainly combines with related receptors and signal molecules through different structural domains to form signal complexes, which have various biological functions, such as participating in the formation of synaptic connections, maintaining synaptic plasticity and participating in pain regulation.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Intracellular Signaling, Phosphoproteins

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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