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Analysis of IL-6R (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0358-LGZ)

Introduction

Interleukin-6 receptor (IL-6R) is the receptor for IL-6 and belongs to the type I cytokine receptor family, subfamily 3. IL-6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. IL-6R is a protein complex consisting of this protein and the interleukin 6 signal transducer (IL6ST/GP130/IL6β), a receptor subunit also shared by many other cytokines. Dysregulated production of IL-6 and this receptor has been implicated in the pathogenesis of many diseases such as multiple myeloma, autoimmune diseases and prostate cancer.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Immunology/Inflammation

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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