Analysis of P-Selectin (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0508-LGZ)

Introduction

P-Selectin (LECAM-3, CD62P) is a member of the selectin family, expressed by activated platelets and endothelial cells. P-selectin binds to a protein called PSGL-1 (P-selectin glycoprotein ligand-1) that appears on the surface of myeloid cells, neutrophils, monocytes, and lymphocytes. P-selectin plays a role in the adsorption of leukocytes and neutrophils to endothelial cells.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Immunology/Inflammation, Oncology & Cancer

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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