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Analysis of RANBP2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1454-LGZ)

Introduction

Official Full Name: RAN binding protein 2<br />Also known as: ANE1; TRP1; TRP2; ADANE; IIAE3; NUP358<br />RAN is a small GTP-binding protein in the RAS superfamily associated with the nuclear membrane and thought to control a variety of cellular functions through interactions with other proteins. This gene encodes a very large ran-binding protein that immunolocalizes to the nuclear pore complex. This protein is a giant scaffold and mosaic cyclophilin-associated nucleoporin that is involved in the Ran-GTPase cycle. The encoded protein directly interacts with the E2 enzyme UBC9 and strongly promotes the transfer of SUMO1 from UBC9 to the SUMO1 target SP100. These findings place polymerization on the cytoplasmic filaments of the nuclear pore complex and suggest that, for some substrates, modification and nuclear import are related events. The gene is partially duplicated in a gene cluster located at a recombination hotspot on chromosome 2q.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements