Analysis of refolded antibody fragments by Differential Scanning Fluorimetry(DSF) (CAT#: STEM-MB-0796-WXH)

Introduction

Fragment antibodies, as the name suggests, are fragments of the binding end of a monoclonal antibody (mAb) and can be a small but mighty tool to fight disease. While mAbs range from around 150kDa in size, fragment antibodies as small as 3kDa have been used in various applications. Refolding is one of the production technologies for pharmaceutical grade antibody fragments.
Differential scanning fluorimetry can be potentially employed as a qualitative measure of functionality, to evaluate refolded proteins, with applicability for antibody engineering in passive immunization.