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Analysis of RPS27 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0640-LGZ)

Introduction

Official Full Name: ribosomal protein S27
Also known as: S27; MPS1; eS27; DBA17; MPS-1
The ribosome is an organelle that catalyzes protein synthesis and consists of a small 40S subunit and a large 60S subunit. These subunits consist of four RNAs and about 80 structurally distinct proteins. This gene encodes a member of the S27e family of ribosomal proteins and a component of the 40S subunit. The encoded protein contains a C4-type zinc finger domain, which can bind zinc and nucleic acid. Mutations in this gene have been found in many melanoma patients and at least one patient with Diamond-Blackfan anemia (DBA). Elevated expression of this gene has been observed in various human cancers. As a typical gene encoding ribosomal proteins, multiple processing pseudogenes of this gene are scattered throughout the genome.

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Principle Applications Procedure Materials

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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