Analysis of SDPR Expression by RT-qPCR (CAT#: STEM-MT-0064-LGZ)

Introduction

Serum deprivation protein response (SDPR) plays an important role in alveolar formation. However, the function and value of SDPR in lung cancer remain unclear. We can evaluate SDPR expression using real-time quantitative PCR (RT-qPCR) on human NSCLC cells, lung adenocarcinoma tissue arrays, kras mutant transgenic mice, TCGA, and GEO datasets.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Lung Cancer

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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