Analysis of SERPING1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2457-LGZ)

Introduction

Official Full Name: serpin family G member 1<br />Also known as: C1IN; C1NH; HAE1; HAE2; C1INH<br />This gene encodes a highly glycosylated plasma protein involved in the regulation of the complement cascade. Its encoded protein, C1 inhibitor, inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. It is synthesized in the liver, and its deficiency is associated with hereditary angioneurotic oedema (HANE). Alternative splicing results in multiple transcript variants encoding the same isoform.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of TMEM210 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1975-LGZ)
Analysis of XRCC2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1848-LGZ)
Analysis of Eperythrozoon Gallians by Real-Time PCR Method (CAT#: STEM-MB-3933-LGZ)
Analysis of Main Drain Virus (MDV) by Real-Time PCR Method (CAT#: STEM-MB-3454-LGZ)
Analysis of Human Herpesvirus-6A (HHV-6A) by Real-Time PCR Method (CAT#: STEM-MB-3326-LGZ)
Analysis of Bacillus Methylotrophicus by Real-Time PCR Method (CAT#: STEM-MB-2809-LGZ)
Analysis of H2AC21 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1024-LGZ)
Analysis of CCDC185 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0836-LGZ)