Analysis of SH2D2A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0149-LGZ)

Introduction

Also known as: SCAP; TSAD; VRAP; F2771<br />This gene encodes an adaptor protein thought to function in T-cell signal transduction. A related protein in mouse is responsible for the activation of lymphocyte-specific protein-tyrosine kinase and functions in downstream signaling. Alternative splicing results in multiple transcript variants.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Hereditary insensitivity to pain with anhidrosis

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of UGT1A4 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1533-LGZ)
Analysis of Macrobrachium Rosenbergii Nodavirus (MrNV) by Real-Time PCR Method (CAT#: STEM-MB-3154-LGZ)
Analysis of NEFL Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1873-LGZ)
Analysis of KLHL30 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1577-LGZ)
Analysis of Human Coronavirus (HCoV-229E) by Real-Time PCR Method (CAT#: STEM-MB-3861-LGZ)
Analysis of PBX1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0563-LGZ)
Analysis of Dichelobacter Nodosus by Real-Time PCR Method (CAT#: STEM-MB-3081-LGZ)
Analysis of YY1AP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0275-LGZ)