Analysis of SPK 1 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0551-LGZ)

Introduction

Sphingosine kinase-1 (SPK1) is a kind of protein kinase which is closely related to the regulation of cell life activities. It plays an important role in cell growth, differentiation and programmed death by regulating sphingosine metabolism balance. The catalytic product of sphingosine 1-phosphate can promote cell proliferation and angiogenesis.

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Principle Applications Procedure Materials Notes Related Products

Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Cardiovascular

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum

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