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Analysis of SRSF7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1747-LGZ)

Introduction

Official Full Name: serine and arginine rich splicing factor 7<br />Also known as: 9G8; AAG3; SFRS7<br />The protein encoded by this gene is a member of the serine/arginine-rich (SR)-rich pre-mRNA splicing factor family that forms part of the spliceosome. Each of these factors contains an N-terminal RNA recognition motif (RRM) for binding RNA and a C-terminal RS domain for binding other proteins. The RS domain is rich in serine and arginine residues that contribute to the interaction between different SR splicing factors. In addition to being essential for mRNA splicing, SR proteins have also been shown to be involved in mRNA export and translation from the nucleus. Multiple transcript variants of this gene have been found encoding different isoforms.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements