Analysis of sTfR-1 (Human) by ELISA (CAT#: STEM-MB-1191-LGZ)

Introduction

The transferrin receptor (TfR) mediates the entry of iron-containing ferritin from the extracellular to the intracellular. TfR is present on the surface of many cells, but it is mostly present in active cells during hemoglobin synthesis. Soluble transferrin receptor (sTfR) is derived by proteolysis of cell surface receptors. sTfR and different transferrin exist in the form of complex in serum. About 80% of sTfR in serum comes from early erythrocytes. When erythropoietic activity increases, especially iron deficiency, sTfR synthesis increases, thus increasing serum sTfR concentration.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Kidney Injury, Toxicology

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma or other biological fluids

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