Unlock Exclusive Discounts & Flash Sales! Click Here to Join the Deals on Every Wednesday!

Analysis of sTNF RI/TNFRSF1A by ELISA (CAT#: STEM-MB-1594-LGZ)

Introduction

Tumor necrosis factor receptor 1 (TNFRI), also known as tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) and CD120a, is a member of the tumor necrosis factor receptor superfamily. It activates the transcription factor NF-κB, mediates apoptosis and regulates inflammation. Mutations in the TNFRI gene are associated with an increased risk of multiple sclerosis. Serum TNFRI levels are elevated in patients with schizophrenia and bipolar disorder, and high levels of TNFRI are associated with more severe psychotic symptoms.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples
Advertisement