Analysis of SULT1C2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1451-LGZ)

Introduction

Official Full Name: sulfotransferase family 1C member 2
Also known as: ST1C1; ST1C2; SULT1C1; humSULTC2
Sulfurtransferases catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs and heterogeneous compounds. These cytoplasmic enzymes differ in tissue distribution and substrate specificity. Gene structure (number and length of exons) was similar among family members. This gene encodes a protein belonging to the SULT1 subfamily responsible for the transfer of the thiol moiety from PAPS to phenolic compounds. Two alternatively spliced transcript variants encoding distinct isoforms have been described for this gene.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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