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Analysis of Syndecan-1 (Human) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0556-LGZ)

Introduction

Syndecan-1 is a member of the adhesion molecule integrin transmembrane binder proteoglycan family. Through the side chain of heparin sulfate on its molecular surface, Syndecan-1 can bind a series of ligands, such as cell adhesion molecules, matrix components, growth factors, enzymes and enzyme inhibitors, to regulate the interaction between cells and the microenvironment in a co-receptor manner. It is involved in the regulation of a series of physiological processes such as the differentiation and development of tissues and organs, angiogenesis and tissue regeneration.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Metabolic, Oncology & Cancer

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
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