Analysis of SYT6 Gene (Mutation) in Hereditary Spastic Paraplegia 47 by RT-qPCR (CAT#: STEM-MT-0128-LGZ)

Introduction

Also known as: sytVI
The protein encoded by this gene belongs to the synaptic protein family. Synaptic tagmins share a common domain structure, including a transmembrane domain and a cytoplasmic region composed of 2 C2 domains, and are involved in calcium-dependent exocytosis of synaptic vesicles. This protein has been shown to be a key component of the secretory machinery involved in acrosomal exocytosis. Alternative splicing transcript variants of this gene have been identified.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Hereditary spastic paraplegia 47, Hoyeraal-Hreidarsson syndrome, Autosomal recessive dyskeratosis congenita

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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