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Analysis of TAF10 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2154-LGZ)

Introduction

Official Full Name: TATA-box binding protein associated factor 10
Also known as: TAF2A; TAF2H; TAFII30
The activity of more than 70 polypeptides is required for RNA polymerase II to initiate transcription. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to properly position the polymerase, serves as a scaffold for the assembly of the rest of the transcription complex, and acts as a channel for regulatory signals. TFIID consists of a TBP-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors (TAFs). TAFs may be involved in basal transcription, acting as coactivators, functioning in promoter recognition or modifying general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a small subunit of TFIID that is associated with a subset of the TFIID complex. Studies in human and mammalian cells have shown that this subunit is required for estrogen receptor transcriptional activation, cell cycle progression, and possibly certain cellular differentiation programs.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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