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Analysis of Thrombospondin-1/THBS-1 by ELISA (CAT#: STEM-MB-1589-LGZ)

Introduction

Thrombospondin-1 (THBS-1) is a member of the thrombospondin family that mediates cell-cell and cell-matrix interactions. This protein binds to fibrinogen, fibronectin, laminin, collagen type V and integrin α-V/β-1. It plays a role in platelet aggregation, angiogenesis and tumorigenesis. In normal tissues, it is a natural inhibitor of neovascularization and tumorigenesis. THBS-1 also positively and negatively regulates endothelial cell adhesion, motility and growth. By blocking the binding of THBS-1 to the cell surface receptor (CD47), normal tissues are almost immune to tumor radiation therapy and contribute to tumor death.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer, Cardiovascular

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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