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Analysis of TL-1A / TNFSF15 / VEGI by ELISA (CAT#: STEM-MB-1559-LGZ)

Introduction

Tumor necrosis factor-like ligand 1A (TL1A), also known as TNFSF15, is a member of the TNF superfamily. It is a homologous trimer cell factor consisting of 251 amino acids expressed by the TNFSF15 gene at locus 9q32 on chromosome 9. Expression of this protein, which is induced by TNF and IL-1α, is highly expressed in endothelial cells but not in B or T cells. This cytokine is a ligand for the TNFRSF25 receptor and the TNFRSF21/DR6 trap receptor. It can activate NF-kappaB and MAP kinases and induce apoptosis of vascular endothelial cells as an autocrine factor. This cytokine also inhibits endothelial cell proliferation and thus may act as an angiogenesis inhibitor.




Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Oncology & Cancer, Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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