Analysis of TNFRSF9 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1243-LGZ)

Introduction

Official Full Name: TNF receptor superfamily member 9<br />Also known as: ILA; 4-1BB; CD137; CDw137; IMD109<br />The protein encoded by this gene is a member of the TNF receptor superfamily. This receptor contributes to the clonal expansion, survival and development of T cells. It can also induce peripheral mononuclear cell proliferation, enhance TCR/CD3 trigger activation-induced T cell apoptosis, and regulate CD28 co-stimulation to promote Th1 cell response. Expression of this receptor is induced by lymphocyte activation. TRAF adapter proteins have been shown to bind to this receptor and transduce signals leading to NF-kappaB activation.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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