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Analysis of TNNI1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0719-LGZ)

Introduction

Official Full Name: troponin I1, slow skeletal type
Also known as: TNN1; SSTNI
Troponin associates with tropomyosin and regulates calcium sensitivity of striated muscle myofibrillar retractors. Troponin I (TnI), together with troponin T (TnT) and troponin C (TnC), is one of three subunits that form the troponin complex in striated muscle filaments. TnI is an inhibitory subunit; blocks actin-myosin interactions, thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-fast skeletal twitch, TnI-slow skeletal twitch, and TnI-cardiac. TnI-fast and TnI-slow genes were expressed in fast-twitch skeletal muscle fibers and slow-twitch skeletal muscle fibers respectively, while TnI-cardiac gene was only expressed in myocardial tissue. This gene encodes troponin-1-skeletal slow-twitch protein. The gene is expressed in early developing heart and skeletal muscle, but only in adult slow-twitch skeletal muscle fibers. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes of the actin-myosin complex.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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