Analysis of Total Oxidant Status (TOS) by Enzyme-labeled Instrument (CAT#: STEM-MB-1449-LGZ)

Under acidic conditions, the oxidizing material in the sample oxidizes Fe2+ to Fe3+, which is highly combined with xylenol orange to produce a blue-purple complex. When the pH of the solution is 2-3, the maximum absorption wavelength is around 590 nm, and the depth of color is proportional to the content of oxide in a certain concentration and a certain time, so as to indirectly measure the total oxidation capacity of the sample.

It is suitable for the detection of total oxidation status in tissue samples, serum and other liquid samples.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: tissue samples, serum and other liquid samples