Analysis of Thiobarbituric Acid Reactants (TBARS) by Enzyme-labeled Instrument (CAT#: STEM-MB-1491-LGZ)

Introduction

Sensitivity:
Detection range:
Precision: inter-lot difference of , intra-lot difference of
Detection equipment: Enzyme-labeled Instrument
Detection wavelength:

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Principle Applications Procedure Materials Notes Related Products

Principle

Thiobarbituric acid reactive substances (TBARS) mainly refers to malondialdehyde, pink compounds that can react with TBA under high temperature and acidic conditions. The concentration of TBARS has a linear relationship with the concentration of Tbars in samples. The concentration of TBARS can be calculated indirectly by measuring OD values at 530-540 nm.

Applications

It is suitable for the determination of TBARS in serum (plasma), animal tissue and other samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum (plasma), animal tissue and other samples

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