Analysis of Albumin (ALB) by Enzyme-labeled Instrument (CAT#: STEM-MB-1373-LGZ)

Introduction

Sensitivity: 0.08 g/L
Detection range: 0.08-15 g/L
Precision: inter-lot difference of 4.6%, intra-lot difference of 4.6%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 620-640 nm

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Principle Applications Procedure Materials

Principle

Bromocresol green (BCG) is a widely used protein staining agent. When the pH value is 4.0-4.2, albumin and bromocresol green are combined, the solution changes from yellow to green, and the color is proportional to the albumin concentration. The albumin content in serum can be calculated by colorimetric comparison.

Applications

It is suitable for the determination of ALB content in serum, plasma and cell supernatant.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum, plasma and cell supernatant

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