Analysis of Lactase Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1422-LGZ)

Introduction

Sensitivity: 3.94 U/ mL
Detection range: 12.5-2000 U/ mL
Precision: inter-lot difference of 8.5%, intra-lot difference of 4.5%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 495 -510 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

Lactase breaks down lactose to produce glucose, which, under the action of the enzyme, produces hydrogen peroxide. In the presence of chromogenic oxygen receptors, peroxidase catalyzes hydrogen peroxide to oxidize pigment sources and produce colored substances.

Applications

It applies to lactase activity in animal tissue samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: animal tissue samples

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