Lactase breaks down lactose to produce glucose, which, under the action of the enzyme, produces hydrogen peroxide. In the presence of chromogenic oxygen receptors, peroxidase catalyzes hydrogen peroxide to oxidize pigment sources and produce colored substances.
Applications
It applies to lactase activity in animal tissue samples.
Procedure
1. Prepare standard samples and experimental samples. 2. Add reaction reagents in order for reaction. 3. Measure the absorbance of each tube. 4. Make the mark curve and calculate the result.