Analysis of Acid Phosphatase (ACP) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1396-LGZ)

Introduction

Sensitivity: 1.40 U/100 mL
Detection range: 1.40-40 U/100 mL
Precision: inter-lot difference of 9.4%, intra-lot difference of 2.1%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 510-530 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

Acid phosphatase can decompose phenylene disodium phosphate under acidic conditions to produce free phenol and phosphoric acid. Phenol reacts with 4-amino-antipyrine in alkaline solution and oxidizes to red quinone derivatives by potassium ferricyanide. The activity of enzyme can be measured according to the shade of red.

Applications

It is used to detect ACP activity of serum (plasma), pleural fluid, urine, cells, cell supernatant and various animal tissues.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum, plasma, urine

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