Analysis of Total Bilirubin (TBIL) by Enzyme-labeled Instrument (CAT#: STEM-MB-1439-LGZ)

Introduction

Sensitivity: 0.7 μmol/L
Detection range: 0.7-50 μmol/L
Precision: inter-lot difference of 4%, intra-lot difference of 2.8%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 565 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

Under the action of accelerators, the hydrogen bond in indirect bilirubin is destroyed, so that the insoluble indirect bilirubin and direct bilirubin react with azo reagent under acidic conditions to produce azo bilirubin. The generated azo bilirubin has the maximum absorption at 565 nm. The content of total bilirubin in serum can be determined by measuring the absorbance change.

Applications

For the determination of total bilirubin in serum of various animals.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum

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