Analysis of Total Cholesterol (TC) by Enzyme-labeled Instrument (CAT#: STEM-MB-1375-LGZ)

Total Cholesterol (TC) includes free cholesterol and cholesterol esters. cholesterol ester can be hydrolyzed by cholesterol esterase (CE) into cholesterol and free fatty acid, and cholesterol was oxidized by cholesterol oxidase (CO) to produce 4-cholestenone and hydrogen peroxide. When hydrogen peroxide is present in 4-amino-antipyrine and phenol, it is catalyzed by oxidase (POD) to generate red quinone compound benzoquinimide phenazone. The color depth is proportional to the content of TC.

It is suitable for the determination of total cholesterol content in serum (plasma) and tissue.

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

• Sample Type: serum (plasma) and tissue