Analysis of β-Hydroxybutyrate (Ketone Body) by Enzyme-labeled Instrument (CAT#: STEM-MB-1461-LGZ)

Introduction

Sensitivity: 0.01 mmol/L
Detection range: 0.01-2.00 mmol/L
Precision: inter-lot difference of 4%, intra-lot difference of 2%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 440-460 nm

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

β-hydroxybutyric acid was oxidized by β-hydroxybutyric acid dehydrogenase, and NAD+ was reduced to generate NADH. NADH made WST-8 orange color under the action of hydrogen-transmitting substances. The content of β-hydroxybutyric acid was determined by the absorbance value measured at 450 nm, and the absorbance value was positively correlated with the content of β-hydroxybutyric acid.

Applications

It is used to detect the content of beta-hydroxybutyric acid in serum, plasma, urine and animal tissue samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum, plasma, urine and animal tissue samples

Other recommended products

Analysis of Albumin (ALB) by Enzyme-labeled Instrument (CAT#: STEM-MB-1373-LGZ)
Analysis of β - galactosidase (β –GAL) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1389-LGZ)
Analysis of Reduced Glutathione (GSH) by Enzyme-labeled Instrument (CAT#: STEM-MB-1498-LGZ)
Analysis of Cell Ferrous Iron by Enzyme-labeled Instrument (CAT#: STEM-MB-1462-LGZ)
Analysis of Creatinine (Cr) by Enzyme-labeled Instrument (CAT#: STEM-MB-1406-LGZ)
Analysis of Sucrose by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1342-LGZ)
Analysis of Aldehyde Dehydrogenase (ALDH) by Fluorescence Microplate Reader (CAT#: STEM-MB-1512-LGZ)
Analysis of Proline (Pro) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1327-LGZ)