Analysis of Total Carbonyl by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1338-LGZ)

Introduction

Sensitivity: 0.94 μg/mL
Detection range: 0.94-45 μg/mL
Precision: inter-lot difference of 8.300000000000001%, intra-lot difference of 4.4%
Detection equipment: Ultraviolet-visible Spectrophotometer
Detection wavelength: 370 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

Carbonyl can react with phenylhydrazine to form a hydrazone, which has a characteristic absorption peak at 370 nm. The content of carbonyl can be calculated by colorimetric determination of absorbance value.

Applications

For the determination of total carbonyl in serum, plasma and animal and plant tissue samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum, plasma and animal and plant tissue samples

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