Myeloperoxidase reduces hydrogen peroxide to a complex, which generates yellow products by hydrogen donor o-anisidine. The product production is measured by colorimetry at 460 nm, so as to calculate the activity of MPO.
Applications
Used to detect MPO activity in serum, plasma, milk, animal tissue and cell samples.
Procedure
1. Prepare standard samples and experimental samples. 2. Add reaction reagents in order for reaction. 3. Measure the absorbance of each tube. 4. Make the mark curve and calculate the result.