Analysis of Hydrogen Peroxide (H2O2) by Fluorescence Microplate Reader (CAT#: STEM-MB-1500-LGZ)

Introduction

Sensitivity: 0.02 μmol/L
Detection range: 0.02-10 μmol/L
Precision: inter-lot difference of 3.6%, intra-lot difference of 1.1%
Detection equipment: Fluorescence Microplate Reader
Excitation wavelength: 535 nm
Emission wavelength: 587 nm

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Principle Applications Procedure Materials Notes Related Products

Principle

H2O2 reacts in the presence of enzyme and fluorescent substance, and its fluorescence intensity at excitation wavelength 535 nm and emission wavelength 587 nm is proportional to the hydrogen peroxide concentration.

Applications

Suitable for detection of hydrogen peroxide content in serum (plasma), animal and plant tissues, cells.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Determination of fluorescence intensity.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum (plasma), animal and plant tissues, cells

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