Analysis of Hydrogen Peroxide (H2O2) by Fluorescence Microplate Reader (CAT#: STEM-MB-1500-LGZ)

Introduction

Sensitivity: 0.02 μmol/L
Detection range: 0.02-10 μmol/L
Precision: inter-lot difference of 3.6%, intra-lot difference of 1.1%
Detection equipment: Fluorescence Microplate Reader
Excitation wavelength: 535 nm
Emission wavelength: 587 nm

Add to Cart Please Inquire

Principle Applications Procedure Materials

Principle

H2O2 reacts in the presence of enzyme and fluorescent substance, and its fluorescence intensity at excitation wavelength 535 nm and emission wavelength 587 nm is proportional to the hydrogen peroxide concentration.

Applications

Suitable for detection of hydrogen peroxide content in serum (plasma), animal and plant tissues, cells.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Determination of fluorescence intensity.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: serum (plasma), animal and plant tissues, cells

Other recommended products

Analysis of Total Antioxidant Capacity (T-AOC) by Enzyme-labeled Instrument (CAT#: STEM-MB-1390-LGZ)
Analysis of Monoamine Oxidase (MAO) by Enzyme-labeled Instrument (CAT#: STEM-MB-1426-LGZ)
Analysis of Lipase (LPS) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1458-LGZ)
Analysis of Copper (Cu) by Enzyme-labeled Instrument (CAT#: STEM-MB-1381-LGZ)
Analysis of Hydrogen Peroxide (H2O2) by Enzyme-labeled Instrument (CAT#: STEM-MB-1371-LGZ)
Analysis of Albumin (ALB) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1320-LGZ)
Analysis of H+K+-ATPase by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1353-LGZ)
Analysis of Acetyl-CoA by Enzyme-labeled Instrument (CAT#: STEM-MB-1475-LGZ)