Analysis of Total Phenols by Enzyme-labeled Instrument (CAT#: STEM-MB-1436-LGZ)

Introduction

Sensitivity: 1.05 μg/mL
Detection range: 1.05–148 μg/mL
Precision: inter-lot difference of 4.4%, intra-lot difference of 4.1%
Detection equipment: Enzyme-labeled Instrument
Detection wavelength: 750-770 nm

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Under alkaline conditions, phenolic substances reduce tungstate molybdate to produce blue compounds. The extraction solution of the test sample has the maximum absorption at 760 nm, and its color depth is proportional to the total phenolic content. The total phenolic content of the sample can be calculated by colorimetry.

Applications

For the determination of total phenolic content in plant tissue samples.

Procedure

1. Prepare standard samples and experimental samples.
2. Add reaction reagents in order for reaction.
3. Measure the absorbance of each tube.
4. Make the mark curve and calculate the result.

Materials

• Sample Type: plant tissue samples

Other recommended products

Analysis of Mitochondrial Complex Ⅴ(F0F1-ATPase/ATP Synthase) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1479-LGZ)
Analysis of Myeloperoxidase (MPO) Activity by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1369-LGZ)
Analysis of Pyruvate Kinase (PK) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1483-LGZ)
Analysis of Phenylalanine Ammonia Lyase (PAL) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1365-LGZ)
Analysis of Creatine kinase (CK) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1347-LGZ)
Analysis of Catalase (CAT) by Ultraviolet-visible Spectrophotometer (CAT#: STEM-MB-1316-LGZ)
Analysis of Lipid Peroxide (LPO) by Enzyme-labeled Instrument (CAT#: STEM-MB-1495-LGZ)
Analysis of Choline Acetyltransferase (ChAT) Activity by Enzyme-labeled Instrument (CAT#: STEM-MB-1427-LGZ)