Analysis of Treponema Hyodysenteriae by Real-Time PCR Method (CAT#: STEM-MB-3198-LGZ)

Introduction

Treponema hyodysenteriae is gram-negative but is more readily observed using the stains listed under Direct Examination. Treponema hyodysenteriae is 6–8.5 μm long, 0.32–0.38 μm in diameter, loosely coiled, motile, cytochrome oxidase negative, catalase negative, stimulated by hydrogen, and anaerobic.<br />A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time), not at its end, as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.