Analysis of TRIM33 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1159-LGZ)

Introduction

Official Full Name: tripartite motif containing 33
Also known as: ECTO; PTC7; RFG7; TF1G; TIF1G; TIFGAMMA; TIF1GAMMA
The protein encoded by this gene is thought to be a transcriptional co-repressor. However, the molecules that interact with this protein have not been identified. This protein is a member of the tripartite motif family. The motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coil region. Three alternatively spliced transcript variants of this gene have been described, however, the full-length nature of one variant has not been determined.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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