Analysis of TSEN15 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0283-LGZ)

Introduction

Official Full Name: tRNA splicing endonuclease subunit 15
Also known as: PCH2F; sen15; C1orf19
This gene encodes a subunit of the tRNA splicing endonuclease, which catalyzes the removal of introns from tRNA precursors. Alternative splicing results in multiple transcript variants. There is a pseudogene of this gene on chromosome 17.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Avian Infectious Bronchitis Virus Ark Type (AIBV-Ark) by Real-Time PCR Method (CAT#: STEM-MB-2862-LGZ)
Analysis of Ureaplasma Spp. by Real-Time PCR Method (CAT#: STEM-MB-3748-LGZ)
Analysis of NOL9 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1351-LGZ)
Analysis of FAM89A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0966-LGZ)
Analysis of EXOC8 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0947-LGZ)
Analysis of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) by Real-Time PCR Method (CAT#: STEM-MB-3274-LGZ)
Analysis of EGR2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2077-LGZ)
Analysis of TMEM81 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0726-LGZ)