Analysis of UBE4B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1435-LGZ)

Introduction

Official Full Name: ubiquitination factor E4B
Also known as: E4; UFD2; HDNB1; UBOX3; UFD2A
Ubiquitin modification of proteins is an important cellular mechanism for the degradation of abnormal or short-lived proteins. Ubiquitination involves at least three classes of enzymes: ubiquitin activating enzymes (E1s), ubiquitin conjugating enzymes (E2s) and ubiquitin protein ligases (E3s). This gene encodes an additional coupling factor, E4, which is involved in polyubiquitin chain assembly. This gene is also the strongest candidate among neuroblastoma tumor suppressor genes. Alternative splice transcript variants of this gene have been found to encode distinct isoforms.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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