Analysis of UCHL5 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0739-LGZ)

Introduction

Official Full Name: ubiquitin C-terminal hydrolase L5<br />Also known as: UCH37; CGI-70; INO80R; UCH-L5<br />Enables endopeptidase inhibitor activity; proteasome binding activity; and thiol-dependent deubiquitinase. Involved in negative regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of smoothened signaling pathway; and protein deubiquitination. Located in cytosol; nucleolus; and nucleoplasm. Colocalizes with Ino80 complex.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of TMEM41B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2150-LGZ)
Analysis of EPS8L2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2252-LGZ)
Analysis of Salmonella Heidelberg by Real-Time PCR Method (CAT#: STEM-MB-3389-LGZ)
Analysis of TM2D2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1882-LGZ)
Analysis of the Q318X Mutation in the CYP21A2 Gene by RT-qPCR (CAT#: STEM-MT-0028-LGZ)
Analysis of Koi Herpesvirus (KHV) by Real-Time PCR Method (CAT#: STEM-MB-3722-LGZ)
Analysis of CRB1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0879-LGZ)
Analysis of F13B Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0173-LGZ)