Unlock Exclusive Discounts & Flash Sales! Click Here to Join the Deals on Every Wednesday!

Analysis of Wnt3a (Human, Mouse, Rat) by MSD Hypersensitive Multi-Factor Electrochemiluminescence Analyzer (CAT#: STEM-MB-0633-LGZ)

Introduction

The Wnt family has multiple secreted glycoproteins that play important roles in animal development. There are 19 Wnt genes in the human genome, which can encode Wnt proteins with different functions. Wnt members bind the Frizzled receptor family of seven transmembrane proteins and activate multiple signal transduction pathways. Aberrant activation of the Wnt signaling pathway is associated with various cancer types. The Wnt3a protein is palmitoylated and acts as a growth factor for hematopoietic stem cells.




Principle

MSD electrochemiluminescence technology is mainly based on ultra-sensitive multi-factor electrochemiluminescence analyzer MES0 Sector600 or Quickplex SQ120. Through dot matrix technology, the detection of 10 indicators per well can be realized in the 96-hole graphite electrode plate. It can also be customized to realize the screening detection of 100 index wells in a 24-well plate.

Applications

Phosphoproteins, Intracellular Signaling

Procedure

1. Coat the capture antibody on the well plate.
2. Add samples/calibrators.
3. Add detection antibody.
4. Read the plate and analyze the data.

Materials

• Sample Type: Plasma, Serum
Advertisement