Antibody Purification by NHS-Activated Magnetic Beads (CAT#: STEM-MB-1309-LGZ)

Introduction

Magnetic bead purification technology has significant advantages over traditional protein and nucleic acid purification techniques. Rapid purification of proteins and antibodies is simple with coated magnetic beads. Save time and money by replacing nucleic acid-based purification columns with silica-coated magnetic bead technology.

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Principle Applications Procedure Materials Notes Related Products

Principle

NHS-Activated MagBeads are pre-activated polymer magnetic microspheres, the surface of which is modified with NHS groups, which can be coupled with amino-containing proteins or polypeptides, and are mainly used for affinity purification of proteins/antibodies.

Applications

For the purification of antibody.

Procedure

1. Buffer preparation.
2. Sample Preparation
3. Bead preparation.
4. Purification of target protein.

Materials

• Washing Buffer
• Elution Buffer
• Neutralization Buffer
• Storage Buffer
• Magnetic Stand or magnet

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