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assess proteins photo-stability by Circular dichroism (CD) (CAT#: STEM-MB-0621-WXH)

Introduction

Among the factors affecting protein stability, light exposure is a major issue for bio-pharmaceutics development. Light exposure leads to denaturation and degradation and eventually loss of activity. The optimisation of a methodology of stability assessment including fast and reproducible photo-stability is therefore a requirement. Light sources with constant and controlled photonflux and with precise and tuneable emission spectrum would be required to ensure reproducibility and reliability.




Principle

Circular dichroism (CD) is a spectroscopy technique that measures the absorption difference between left and right circularly polarized light. By symmetry, this asymmetric absorption can only occur for asymmetric molecules, meaning chiral molecules.

Applications

Circular dichroism (CD) spectroscopy is a powerful technique that is sensitive to the chirality (handedness) of molecules. It can be used to study absolute stereochemistry, enantiomeric composition, racemization, enantiomeric differentiation, and molecular interactions and conformation.

Procedure

1. Sample preparation
2. Measurement by CD instrument
3. Data analysis

Materials

Circular dichroism (CD) spectrophotometer
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